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intensilight epifluorescence source  (Nikon)


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    Structured Review

    Nikon intensilight epifluorescence source
    Intensilight Epifluorescence Source, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intensilight+epifluorescence+source/Intensilight/pm24417287__ja411339f_si_001-85-13-16
    Average 96 stars, based on 1024 article reviews
    intensilight epifluorescence source - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Light-responsive Polymer Particles as Force Clamps for Mechanical Unfolding of Target Molecules
    Article Snippet: The galvo system was integrated with a Nikon Eclipse Ti microscope driven by the Elements software package. .. The microscope features an Evolve electron multiplying charge coupled device (EMCCD; Photometrics), an Intensilight epifluorescence source (Nikon), a Nikon CFI S Fluor 100x oil objective (numerical aperture (NA) 1.49) (Nikon), a Chroma FITC excitation filter set, a Chroma TRITC excitation filter set, a Chroma TIRF561 excitation filter set and a Chroma Cy5 excitation filter set. ..

    Article Title: Supplementary information: Quantum dots encapsulated within phospholipid membranes: phase-dependent structure, photostability, and site-selective functionalization
    Article Snippet: The microscope was Nikon Eclipse Ti driven by the Elements software package. .. The microscope features an Evolve electron multiplying charge coupled device (CCD; Photometrics), an Intensilight epifluorescence source (Nikon), a CFI Apo 100× (numerical aperture (NA) 1.49) objective (Nikon) and a TIRF launcher with two laser lines: 488 nm (10 mW) and 638 nm (20 mW). .. This microscope also includes the Nikon Perfect Focus System, an interferometry-based focus lock that allowed the capture of multipoint and time-lapse images without loss of focus.

    Article Title: Supporting Information: Titin-Based Nanoparticle Tension Sensors Map High- Magnitude Integrin Forces within Focal Adhesions
    Article Snippet: A Nikon Eclipse Ti microscope driven by the Elements software package was used for imaging. .. The microscope is equipped with a TIRF launcher with three laser lines: 488 nm (10 mW), 561 (50 mW), and 638 nm (20 mW), an intensilight Epifluorescence source (Nikon), an Evolve electron multiplying charge coupled device (EMCCD camera, Photometrics), and a CFI Apo 100x (NA 1.49) objective (Nikon). ..

    Article Title: Force-Induced Site-Specific Enzymatic Cleavage Probes Reveal That Serial Mechanical Engagement Boosts T Cell Activation
    Article Snippet: Epi fluorescence microscopy and total internal reelection fluorescence (TIRF) microscopy experiments were performed on a Nikon Eclipse Ti inverted microscope driven by the NIS Elements software. .. The microscope features an Evolve electron-multiplying charge-coupled device (Photometrics), an Intensilight epifluorescence source (Nikon), CFI Apo 100 X (numerical aperture 1.49) objective and 40 X (numerical aperture 0.75) objective (Nikon), and a total internal reflection fluorescence launcher with three laser lines: 488 (10 mW), 561 (50 mW), and 638 nm (20 mW). ..

    Article Title: Molecular Compressive Force Sensor for Mapping Forces at the Cell–Substrate Interface
    Article Snippet: .. The microscope used was NSTORM Nikon microscope (Ti E motorized inverted microscope body), operated by Nikon Elements software, equipped with an Intensilight epifluorescence source (Nikon), a CFI Apo 100X NA 1.49 objective was used. .. All ultrapure water was obtained from a Barnstead Nanopure water purifying system (Thermo Fisher) that indicated a resistivity of 18.2 MΩ.

    Article Title: Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of integrin clustering on force transmission
    Article Snippet: The microscope was a Nikon Eclipse Ti driven by the Elements software package. .. The microscope features an Evolve electron multiplying charge coupled device (EMCCD; Photometrics), an Intensilight epifluorescence source (Nikon), a CFI Apo 100× (numerical aperture (NA) 1.49) objective (Nikon) and a TIRF launcher with two laser lines: 488 nm (10 mW) and 647 nm ( 20 mW). .. This microscope also includes the Nikon Perfect Focus System, an interferometry-based focus lock that allowed the capture of multipoint and time-lapse images without loss of focus.

    Article Title: Supporting Information
    Article Snippet: .. Images were acquired using a Nikon Eclipse Ti epifluorescence microscope equipped with a TIRF launcher with three laser lines: 488 nm (10 mW), 561 nm (50 mW), and 638 nm (20 mW), an Intensilight epifluorescence source, a EMCCD camera (Photometrics) and the following Chroma filter cubes: reflection interference contrast microscopy (RICM), TIRF 488, TIRF 561 and TIRF 640, and a CFI Apo x100 objective (Nikon, numerical aperture = 1.49). .. Images were captured using the Element software package (Nikon) with the following exposure times: 50–100 ms for RICM channel; 50–300 ms for T561 channel and 50–100 ms for T488 channel.

    Article Title: Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors.
    Article Snippet: The T cell receptor (TCR) is thought to be a mechanosensor, meaning that it transmits mechanical force to its antigen and leverages the force to amplify the specificity and magnitude of TCR signalling.. Although a variety of molecular probes have been proposed to quantify TCR mechanics, these probes are immobilized on hard substrates, and thus fail to reveal fluid TCR–antigen interactions in the physiological context of cell membranes.. Here we developed DNA origami tension sensors (DOTS) which bear force sensors on a DNA origami breadboard and allow mapping of TCR mechanotransduction at dynamic intermembrane junctions.

    Fluorescence:

    Article Title: Force-Induced Site-Specific Enzymatic Cleavage Probes Reveal That Serial Mechanical Engagement Boosts T Cell Activation
    Article Snippet: Epi fluorescence microscopy and total internal reelection fluorescence (TIRF) microscopy experiments were performed on a Nikon Eclipse Ti inverted microscope driven by the NIS Elements software. .. The microscope features an Evolve electron-multiplying charge-coupled device (Photometrics), an Intensilight epifluorescence source (Nikon), CFI Apo 100 X (numerical aperture 1.49) objective and 40 X (numerical aperture 0.75) objective (Nikon), and a total internal reflection fluorescence launcher with three laser lines: 488 (10 mW), 561 (50 mW), and 638 nm (20 mW). ..

    Article Title: Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors.
    Article Snippet: The T cell receptor (TCR) is thought to be a mechanosensor, meaning that it transmits mechanical force to its antigen and leverages the force to amplify the specificity and magnitude of TCR signalling.. Although a variety of molecular probes have been proposed to quantify TCR mechanics, these probes are immobilized on hard substrates, and thus fail to reveal fluid TCR–antigen interactions in the physiological context of cell membranes.. Here we developed DNA origami tension sensors (DOTS) which bear force sensors on a DNA origami breadboard and allow mapping of TCR mechanotransduction at dynamic intermembrane junctions.

    Inverted Microscopy:

    Article Title: Molecular Compressive Force Sensor for Mapping Forces at the Cell–Substrate Interface
    Article Snippet: .. The microscope used was NSTORM Nikon microscope (Ti E motorized inverted microscope body), operated by Nikon Elements software, equipped with an Intensilight epifluorescence source (Nikon), a CFI Apo 100X NA 1.49 objective was used. .. All ultrapure water was obtained from a Barnstead Nanopure water purifying system (Thermo Fisher) that indicated a resistivity of 18.2 MΩ.

    Software:

    Article Title: Molecular Compressive Force Sensor for Mapping Forces at the Cell–Substrate Interface
    Article Snippet: .. The microscope used was NSTORM Nikon microscope (Ti E motorized inverted microscope body), operated by Nikon Elements software, equipped with an Intensilight epifluorescence source (Nikon), a CFI Apo 100X NA 1.49 objective was used. .. All ultrapure water was obtained from a Barnstead Nanopure water purifying system (Thermo Fisher) that indicated a resistivity of 18.2 MΩ.



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